LIPID PEROXIDATION OF BIOLOGICAL MEMBRANES : EFFECT OF ANTIOXIDANTS

Coordinator: Dr. Angel Catalá

Our laboratory has been interested for many years on the lipid peroxidation of biological membranes from various tissues and different species. The process of lipid peroxidation is followed by the use of modern techniques to measure light emission: chemiluminescence produced during the formation of alkoxyl radicals formed during the lipid peroxidation of long chain unsaturated fatty acids present mainly in phospholipids of biological membranes. We have tested the effect of many antioxidants such as: α-tocopherol, vitamin A, melatonin and acetyl serotonin among others. For a detailed review see (An overview of lipid peroxidation with emphasis in outer segments of photoreceptors and the chemiluminescence assay Review Angel Catalá Int J Biochem Cell Biol 2006 38: 1482-1495)

Research Staff

Natalia S. Fagali

Selected publications in the frame of this project (2007 - 2008)

* “Arachidonic acid hydroperoxide stimulates lipid peroxidation on rat liver nuclei and chromatin fractions”.
M. Marmunti y A. Catalá
Molecular and Cellular Biochemistry 298, 161-168 (2007).

* “Non enzymatic lipid peroxidation of microsomes and mitochondria from liver, heart and brain of the bird Lonchura Striata : relationship with fatty acid composition”.
A.M. Gutierrez, G. Reboredo, S.M. Mosca y A. Catalá
Comparative Biochemistry and Physiology A-Molecular and Integrative Physiology 146, 415-421 (2007).

* “The effect of melatonin and structural analogues on the lipid peroxidation of triglycerides enriched in ?-3 polyunsaturated fatty acids”.
N. Fagali y A. Catalá
Life Sciences 81, 299-305 (2007).

* “Melatonin and n-acetyl serotonin inhibit selectively enzymatic and non-enzymatic lipid peroxidation of rat liver microsomes”.
P.J. Leaden y A. Catalá
Prostaglandins Leukot Essent Fatty Acids 77, 29-35 (2007).

* “The ability of melatonin to counteract lipid peroxidation in biological membranes”.
A. Catalá
Current Molecular Medicine 7, 638-649 (2007).

* “Antioxidant activity of conjugated linoleic acid isomers, linoleic acid and its methyl ester determined by photoemission and DPPH· techniques”.
N. Fagali y A. Catalá
Biophys Chem 137, 56-62 (2008).

* “An allometric study of fatty acids and sensitivity to lipid peroxidation of brain microsomes and mitochondria isolated from different bird species”.
A.M. Gutierrez, G. Reboredo, S.M. Mosca y A. Catalá
Molecular and Integrative Physiology 150, 359-365 (2008).